Fluorescence Microscopes LOT 1: Confocal Microscope

… enable high-resolution multidimensional and spectral imaging as well as the investigation of dynamic cellular processes. Among other things, multi-color and high-speed imaging, fluorescence lifetime measurements (FLIM), FRET/FRAP applications as well as the recording and evaluation of 3D and time series data are planned. The system should have flexible excitation and spectral detection capabilities and be suitable for quantitative and functional fluorescence imaging. The associated software is intended to enable the control, recording, processing, quantification and evaluation of multidimensional image data. On behalf of the Faculty of Medicine, the following procurement is carried out: As part of the research project ‘KOMPASS – Combined Platform for Substance and System Development’, the microscopic research infrastructure is to be expanded by two high-performance systems for high-resolution and three-dimensional fluorescence imaging. The subject of the award is the supply, installation and commissioning of a confocal laser scanning microscope and a light sheet microscope, including the necessary hardware and software components, image acquisition and evaluation software, computer systems, accessories and instruction or application training. The contract will be awarded in two lots. Lot 2 – Light sheet microscope Procurement of a light sheet microscope for fast three-dimensional fluorescence imaging of biological samples. The system is particularly suitable for the gentle volumetric imaging of living 2D and 3D cell cultures, tissue samples and other biological model systems as well as for long-term and high-throughput experiments. A system for the rapid acquisition of three-dimensional image data with multichannel fluorescence imaging, automated sample and light sheet alignment as well as possibilities for the use of different sample carriers including slides, cell culture trays and multiwell plates is required. The necessary image acquisition and evaluation software as well as the computer infrastructure required for the processing of extensive 3D data sets are also part of the system.